Abstract: Objective To study the expression of hK2 and Ki-67 in prostate cancer and its clinical significance. Methods Expression of hK2 and Ki-67 was studied in tissues collected from 40 cases of prostate cancer（PCa） and 30 cases of benign prostatic hyperplasia（BPH） using the immunohistochemical SP method. Results The rates of hK2 and Ki-67 expression were significantly higher in PCa than in BPH （P<0.05）.Positive expression of hK2 in PCa tissue correlated with Gleason grading （P<0.05） but not with clinical stage（P>0.05），while positive expression of Ki-67 correlated with clinical stage（P<0.05） but not with Gleason grading（P>0.05）.Expression of hK2 positively correlated with expression of Ki-67（P<0.05）. Conclusions Abnormal expression of hK2 and Ki-67 correlated closely with the diagnosis and prognosis of PCa.Combined detection of hK2 and Ki-67 may be useful as a marker for early diagnosis and prognostic evaluation of PCa.

Key words: hK2, Ki-67, Prostate cancer, Benign prostatic hyperplasia